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We report a microwave spectroscopy measurement of the muonium fine structure transition, yielding a transition frequency of , in agreement with state-of-the-art QED predictions within one standard deviation. In combination with the recent Lamb shift result, the splitting is determined, improving the spectroscopic characterization of the manifold in muonium. These results provide a stringent test of bound-state QED in a purely leptonic system and establish a path toward future searches for Lorentz violation and muon-specific new physics.

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