Let be a digraph with vertex set and be digraphs. The generalized join digraph is a digraph obtained from by replacing each vertex with and for any and , if and only if . In this paper we express the number of oriented spanning trees in in terms of Laplacian eigenvalues of and oriented spanning trees of . Furthermore, we consider the number of oriented spanning trees with a fixed root in . First, we introduce the biclique-directed star transformation formula for counting oriented spanning trees with a fixed root in digraphs. Using it, we give the formula for the total number of oriented spanning trees with roots in a certain of in terms of Laplacian eigenvalues of and oriented spanning trees of . As applications, when each is a given digraph, the enumerative formulas for oriented spanning trees with a fixed root of are derived from our work.

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