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Regular nilpotent Hessenberg varieties form an important family of subvarieties of the flag variety, which are often singular and sometimes not normal varieties. Like Schubert varieties, they contain distinguished points called permutation flags. In this paper, we give a combinatorial characterization for a permutation flag of a regular nilpotent Hessenberg variety to be a singular point. We also apply this result to characterize regular nilpotent Hessenberg varieties which are normal algebraic varieties.

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