A search for pair-produced vector-like quarks using events with exactly one lepton ( or ), at least four jets including at least one -tagged jet, and large missing transverse momentum is presented. Data from proton-proton collisions at a centre-of-mass energy of TeV, recorded by the ATLAS detector at the LHC from 2015 to 2018 and corresponding to an integrated luminosity of 139 fb, are analysed. Vector-like partners and of the top and bottom quarks are considered, as is a vector-like with charge +5/3, assuming their decay into a , , or Higgs boson and a third-generation quark. No significant deviations from the Standard Model expectation are observed. Upper limits on the production cross-section of and quark pairs as a function of their mass are derived for various decay branching ratio scenarios. The strongest lower limits on the masses are 1.59 TeV assuming mass-degenerate VLQs and branching ratios corresponding to the weak-isospin doublet model, and 1.47 TeV (1.46 TeV) for exclusive () decays. In addition, lower limits on the and quark masses are derived for all possible branching ratios.

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