In this paper, we perform chiral perturbative analysis of an approximate lepton number symmetry associated with a sufficiently light neutrino in the type-I seesaw mechanism. For the Dirac mass matrix , linearly independent components of from and are treated as symmetry-breaking parameters. A deviation in the eigenvector of the massless mode occurs in the first-order perturbation and the lightest mass emerges in the second-order. By solving the perturbation theory, we obtained specific expressions of and . As a result, two complex parameters in are bounded to some extent from the eigenvector. These constraints are associated with the chiral symmetry and are not susceptible to renormalization.

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