Let with and . Let be the discrete periodic Schrödinger operator on , where is the discrete Laplacian and is -periodic. In this paper, we develop tools from complex analysis to study the isospectrality of discrete periodic Schrödinger operators. We prove that if two -periodic potentials and are Fermi isospectral and both and are separable functions, then, up to a constant, one dimensional potentials and are Floquet isospectral, . This allows us to prove that for any non-constant separable real-valued -periodic potential, the Fermi variety is irreducible for any , which partially confirms a conjecture of Gieseker, Knörrer and Trubowitz in the early 1990s.

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