We report the results of first ever spectro-polarimetric analyses of the Galactic ultra-luminous X-ray pulsar Swift J0243.6 during the 2023 outburst using quasi-simultaneous {\it IXPE}, {\it NICER} and {\it NuSTAR} observations. A pulsation of period s is detected in {\it IXPE} and {\it NuSTAR} observations with pulse fractions (PFs) ( keV) and ( keV), respectively. Energy dependent study of the pulse profiles with {\it NuSTAR} indicates an increase in PF from ( keV) to ( keV). Further, epoch-dependent polarimetric measurements during the decay phase of the outburst confirm the detection of significant polarization, with the polarization degree (PD) and polarization angle (PA) ranging between and , respectively, in the keV energy range. We also observe that the PD increases up to at higher energies ( keV) with dominating flux contribution () in the {\it IXPE} spectra. The phase-resolved polarimetric study yields PD as suggesting a marginal correlation with the pulse profiles. Moreover, the broad-band ( keV) energy spectrum of combined {\it NICER} and {\it NuSTAR} observations is well described by the combination of and components with seed photon temperature () keV and photon index () . With the above findings, we infer that the observed low' PD in Swift J0243.6$+6124$ is attributed possibly due to vacuum resonance’ effect between the overheated and relatively cooler regions of the neutron star boundary layer.

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