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We compute the signature of the Milnor fiber of certain type of non-isolated complex surface singularities, namely, images of finitely determined holomorphic germs. An explicit formula is given in algebraic terms. As a corollary we show that the signature of the Milnor fiber is a topological invariant for these singularities. The proof combines complex analytic and smooth topological techniques. The main tools are Thom-Mather theory of map germs and the Ekholm-Szűcs-Takase-Saeki formula for immersions. We give a table with many examples for which the signature is computed using our formula.

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