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We study the Lane-Emden problem where is a smooth bounded domain and is sufficiently large. We obtain sharp estimates and non-degeneracy of low energy nodal solutions (i.e. nodal solutions satisfying ). As applications, we prove that the comparable condition holds automatically for least energy nodal solutions, which confirms a conjecture raised by (Grossi-Grumiau-Pacella, Ann.I.H. Poincare-AN, 30 (2013), 121-140) and (Grossi-Grumiau-Pacella, J.Math.Pures Appl. 101 (2014), 735-754).

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