A search for a dark baryon is performed for the first time in the two-body decay using events collected at a center-of-mass energy of with the BESIII detector at the BEPCII collider. No significant signal is observed, and the 90% (95%) confidence level upper limits on the branching fraction are determined to be (), (), (), () and (), under the dark baryon mass hypotheses of 1.07, 1.10, (1.116), 1.13, and 1.16, respectively. The constraints obtained on the Wilson coefficients and are more stringent than the previous limits derived from the LHC searches for the colored mediators.

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