We report the discovery of an isolated millisecond pulsar M15O (J2129+1210O) from the globular cluster M15 (NGC 7078) with a period of 11.06686 ms and a dispersion measure of 67.44 cm pc. Its spin period is so close to the 10th harmonic of the bright pulsar M15A (11.06647 ms) that it was missed in previous pulsar search. We suggest adding the spectrum in the pulsar candidate diagnostic plot to identify new signals near the harmonics. M15O has the first spin frequency derivative and the second spin frequency derivative,being 1.79191(5) Hz and 3.3133(6) Hz , respectively. Its projected distance from the optical center of M15 is the closest among all the pulsars in M15. The origin can be something from the center of the massive and core-collapsed globular cluster M15.

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