We study the uniqueness and nondegeneracy of positive bubble solutions for the generalized energy-critical Hartree equation on the Heisenberg group , \begin{equation}\label{0.1} -Δ_{}u=\left(\int_{^{n}}\frac{|u(η)|^{Q^{\ast}_μ}}{|η^{-1}ξ|^μ}η\right)|u|^{Q^{\ast}μ-2}u,~~~ξ,η\in^{n}, \end{equation} where represents the Kohn Laplacian, is a real-valued function, is the homogeneous dimension of , is a real parameter and is the upper critical exponent following the Hardy-Littlewood-Sobolev inequality on the Heisenberg group. By applying the Cayley transform, the spherical harmonic decomposition and the Funk-Hecke formula of the spherical harmonic function, we prove the nondegeneracy of positive bubble solutions for (\ref{0.1}). As an applications, we investigate the asymptotic behavior of the solutions for the Brezis-Nirenberg type problem as \begin{equation}\label{0.2} \left{ \begin{aligned} &-Δ{}u=\varepsilon u+\left(\int_Ω\frac{|u(η)|^{Q^{\ast}_μ}}{|η^{-1}ξ|^μ}η\right)|u|^{Q^{\ast}_μ-2}u,&&~Ω\subset ^{n}, &u=0,&&~\partialΩ. \end{aligned} \right. \end{equation}

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