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This is the second paper in a series on {\it Virasoro constraints for Cohomological Field Theory}. We derive the ancestor Virasoro constraints for the topological recursion (TR) for an arbitrary spectral curve and establish the descendent Virasoro constraints for spectral curves satisfying certain conditions. For higher-genus curves, we further establish the corresponding ancestor and descendent Virasoro constraints for the associated non-perturbative generating series. We present several examples that illustrate the comparison between the descendent Virasoro constraints for TR descendent invariants and the original Virasoro constraints for geometric descendent invariants.

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