A set of spanning trees in a graph is called a set of if, for every pair of vertices and , the paths connecting and across different trees do not share any vertices or edges, except for and themselves. Hasunuma conjectured that every -connected graph contains exactly completely independent spanning trees (CISTs). However, Pétérfalvi disproved this conjecture. When , the two CISTs are called a . It has been shown that determining whether a graph can have CISTs is an NP-complete problem, even when . In , Darties et al. raised the question of whether the dimensional hypercube can have three completely independent spanning trees (CISTs). This paper provides an answer to that question. In this paper, we first present a necessary condition for -regular, -connected bipartite graphs to have CISTs. We also investigate that the hypercube of dimension cannot have CISTs, which means Hasunuma’s conjecture does not hold for the hypercube when is an even integer , except when and . This result also resolves a question posed by Darties et al. The construction of multiple CISTs on the underlying graph of a network has practical applications in ensuring the fault tolerance of data transmission. In this context, we also provide a construction for three completely independent spanning trees in the hypercube for . Our results show that Hasunuma’s conjecture holds for odd integer in , but does not hold for even integer .

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