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An experimental indication of negative heat capacity in excited nuclear systems is inferred from the event by event study of energy fluctuations in quasi-projectile sources formed in collisions at 35 A.MeV. The excited source configuration is reconstructed through a calorimetric analysis of its de-excitation products. Fragment partitions show signs of a critical behavior at about 5 A.MeV excitation energy. In the same energy range the heat capacity shows a negative branch providing a direct evidence of a first order liquid gas phase transition.

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