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We define a Floer-homology invariant for knots in an oriented three-manifold, closely related to the holomorphic disk Floer homologies for three-manifolds defined in an earlier paper. We set up basic properties of these invariants, including an Euler characteristic calculation, behaviour under connected sums. Then, we establish a relationship with for surgeries along the knot. Applications include calculation of of three-manifolds obtained by surgeries on some special knots in , and also calculation of for certain simple three-manifolds which fiber over the circle.

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