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We develop a general gauge invariant construction of the one-loop effective action for supersymmetric gauge field theories formulated in superspace. Using manifestly covariant techniques (the background superfield method and proper-time representations) adopted to the superspace we show how to define unambiguously the effective action of a matter multiplet (in fundamental and adjoint representations) and the vector multiplet coupled to a background gauge superfield. As an application of this construction we exactly calculate the low-energy one-loop effective action of matter multiplet and SU(2) SYM theory on the Abelian background.

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