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Let be the eigenavalues of matrix. In this paper, we characterize connected graphs with eigenvalue . As a result, we determine all connected graphs with exactly two distinct eigenvalues. We show that a connected bipartite graph has three distinct eigenvalues if and only if it is a complete bipartite graph. Furthermore, we present some bounds for the spectral radius (resp. energy) and characterize extremal graphs. Also, we obtain a relation between energy and energy. Finally, the chemical importance of energy is investigated and it shown that the energy is useful in predicting certain properties of molecules.

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