I point out that decays are sensitive probes of possible violation of CKM unitarity. I compute the decay rates and asymmetries in a minimal extension of the Standard Model containing an additional isosinglet charge (-1/3) quark, which leads to a deviation from CKM unitarity. It is shown that even for small mixing ratios , the contribution of the tree-level FCNC appearing in the model should change the rates and asymmetries significantly. Especially the CP asymmetry, $A_{CP} (B \to X_s \l^+ \l^-)$, can be enhanced to be few percents, while in the standard model the size is less than . On the other hand, $A_{CP} (B \to X_d \l^+ \l^-)$ is not altered so much. Constraints for the mixing ratios are extracted from the experiments of for and $B_d^0 - \bar{B}d^0q = d$ under a natural assumption that the couplings of the tree-level are almost unity, i.e. $z{\alpha \alpha} \sim 1$.

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