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We study the pion structure function in nuclear medium using the Nambu and Jona-Lasinio model, and its implication for the nuclear pion enhancement of the sea quark distribution in nuclei. By using the operator product expansion, medium effect of the nuclear matter is incorporated in calculations of the twist-2 operators. We find density dependence of the pion structure function is rather weak around the nuclear matter density. We also discuss how the medium modification of the pion structure affects the sea quark enhancement in the nucleus.

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