We investigate the twist-3 effects in the polarized virtual photon structure. The structure functions and of polarized photon could be experimentally studied in the future polarized or colliders. The leading contributions to are the twist-2 effects, while another structure function , which only exists for the virtual photon target, receives not only the twist-2 but also twist-3 contributions. We first show that the twist-3 effects actually exist in the box-diagram contributions and we extract the twist-3 part, which can also be reproduced by the pure QED operator product expansion. We then calculate the non-trivial lowest moment () of the twist-3 contribution to in QCD. For large (the number of colors), the QCD analysis of the twist-3 effects in the flavor nonsinglet part of becomes tractable and we can obtain its moments in a compact form for all .

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