Recent studies have shown that the star formation rate (SFR) correlates tightly and linearly with the mass of gravitationally bound gas, which can be delineated from the power-law tail of the column-density probability distribution function (-PDF) derived from dust emission observations. This relationship holds across four orders of magnitude within the Milky Way–spanning low-mass to high-mass star-forming regions and encompassing the extreme environment of the Central Molecular Zone. Building on this framework, we present a new approach for estimating the mass of gravitationally bound gas in molecular clouds using multi-line CO isotopologue observations. Our sample includes 16 molecular clouds with robust detections in CO, CO, and CO = 1-0, spanning both massive inner Galaxy clouds and nearby star-forming regions. We find that the -PDFs derived from combined CO isotopologue data recover the characteristic log-normal plus power-law profiles seen in dust-based studies. The mass and spatial distribution of the self-gravitating structures estimated from both dust-based and CO-based methods agree well throughout the sample. This indicates that the CO isotopologue combination can robustly trace the self-gravitating component via the -PDF method and provides a reliable, scalable, and velocity-resolved alternative to dust emission for identifying the star-forming gas in molecular clouds.

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