We study the instanton effect on the gluon scattering amplitudes at strong coupling and large for the supersymmetric Yang-Mills theory. According to Alday and Maldacena, the gluon scattering amplitude corresponds holographically to the area of a worldsheet minimal surface in the T-dual AdS geometry. The Yang-Mills instanton introduces an instanton D-brane in the geometry, with which a particular boundary condition for the minimal surface is imposed. We show that the minimal surface undergoes a topology change depending on the size and the gluon momenta, and that the instanton amplitude exhibits a characteristic dependence on gluon momenta. More specifically, we find that when the fixed instanton size modulus is larger than where is the 't Hooft coupling and is the typical momentum of the scattering gluon, due to the topology change of the worldsheet minimal surface, the instanton amplitude is exponentially enhanced as .

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