In the present work, motivated by the studies on the low Mach number limit problem, we establish uniform regularity estimates with respect to the Mach number for the non-isentropic compressible Navier-Stokes system in smooth domains with Navier-slip boundary conditions, in the general case of ill-prepared initial data. The thermal conduction is taken into account and the large variation of temperature is allowed. Moreover, the obtained regularity estimates are also uniform in the Reynolds number P'eclet number provided where is a fixed constant independent of the Mach number, Reynolds number, and P'eclet number. The large temperature variation as well as the interactions of two kinds of boundary layers are the main obstacles to the proof.

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