Bigness of the tangent bundle of a Fano threefold with Picard number two

In this paper, we study the positivity property of the tangent bundle $T_X$ of a Fano threefold $X$ with Picard number 2. We determine the bigness of the tangent bundle of the whole 36 deformation types. Our result shows that $T_X$ is big if and only if $(-K_X{)}^3\ge 34$. As a corollary, we prove that the tangent bundle is not big when $X$ has a standard conic bundle structure with non-empty discriminant. Our main methods are to produce irreducible effective divisors on $\mathbb{P}(T_X)$ constructed from the total dual VMRT associated to a family of rational curves. Additionally, we present some criteria to determine the bigness of $T_X$.