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In this paper, we prove a quantitative regularity theorem and a blow-up criterion of classical solutions for the three-dimensional Navier-Stokes equations. By adapting the strategy developed by Tao in [20], we obtain an explicit blow-up rate in the setting of critical Lorentz spaces $L^{3, q_{0}}(\mathbb R^3)3 \leq q_0 < \infty $. Our results improve the previous regularity in critical Lebesgue spaces in [20] and quantify the qualitative result by Phuc in [16].

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