Separative von Neumann regular rings exist in abundance. For example, all regular self-injective rings, unit regular rings, regular rings with a polynomial identity are separative. It remains open whether there exists a non-separative regular ring. In this note, we study a variety of conditions under which a von Neumann regular ring is separative. We show that a von Neumann regular ring is separative under anyone of the following cases: {\it (1)} is CS; {\it (2)} is pseudo injective (auto-injective); {\it (3)} satisfies the closure extension property: the essential closures in of two isomorphic right ideals are themselves isomorphic. We also give another characterization of a regular perspective ring (Proposition 3.3)

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