We present a uniqueness theorem for the reduced C*-algebra of a twist over a Hausdorff 'etale groupoid . We show that the interior of the isotropy of is a twist over the interior of the isotropy of , and that the reduced twisted groupoid C*-algebra embeds in . We also investigate the full and reduced twisted C*-algebras of the isotropy groups of , and we provide a sufficient condition under which states of (not necessarily unital) C*-algebras have unique state extensions. We use these results to prove our uniqueness theorem, which states that a C*-homomorphism of $C_r^(; )C_r^(^; ^)$ is injective. We also show that if is effective, then is simple if and only if is minimal.

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