Nikiforov [Some inequalities for the largest eigenvalue of a graph, Combin. Probab. Comput. 179–189] showed that if is -free then the spectral radius , which implies that contains if . In this paper, we follow this direction on determining which subgraphs will be contained in if , where as . We first show that if $\rho(G)\geq \sqrt{m}GK_{2,r+1}GG$ contains either or unless is a complete bipartite graph, where denotes the graph obtained from and by identifying an edge. Secondly, we prove that if , then contains pentagon and hexagon unless is a book; and if , then contains for every . In the end, some related conjectures are provided for further research.

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