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In this paper, we first introduce the concept of symmetric biderivation radicals and characteristic subalgebras of Lie algebras, and study their properties. Based on these results, we precisely determine biderivations of some Lie algebras including finite-dimensional simple Lie algebras over arbitrary fields of characteristic not or , and the Witt algebras over fields of characteristic . As an application, commutative post-Lie algebra structure on aforementioned Lie algebras is shown to be trivial.

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