We define two concordance invariants of knots using framed instanton homology. These invariants and provide bounds on slice genus and maximum self-linking number, and the latter is a concordance homomorphism which agrees in all known cases with the invariant in Heegaard Floer homology. We use and to compute the framed instanton homology of all nonzero rational Dehn surgeries on: 20 of the 35 nontrivial prime knots through 8 crossings, infinite families of twist and pretzel knots, and instanton L-space knots; and of 19 of the first 20 closed hyperbolic manifolds in the Hodgson–Weeks census. In another application, we determine when the cable of a knot is an instanton L-space knot. Finally, we discuss applications to the spectral sequence from odd Khovanov homology to the framed instanton homology of branched double covers, and to the behaviors of and under genus-2 mutation.

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