We present results from a lattice QCD study of nucleon matrix elements at vanishing momentum transfer for local and twist-2 isovector operator insertions. Computations are performed on gauge ensembles with non-perturbatively improved Wilson fermions, covering four values of the lattice spacing and pion masses down to MeV. Several source-sink separations (typically ~1.0fm to ~1.5fm) allow us to assess excited-state contamination. Results on individual ensembles are obtained from simultaneous two-state fits across all observables and all available source-sink separations with the energy gap as a common fit parameter. Renormalization has been performed non-perturbatively using the Rome-Southampton method for all but the finest lattice spacing for which an extrapolation has been used. Physical results are quoted in the scheme at a scale of GeV and are obtained from a combined chiral, continuum and finite-size extrapolation. For the nucleon isovector axial, scalar and tensor charges we find physical values of , and , respectively, where individual systematic errors in each direction from the chiral, continuum and finite-size extrapolation have been added in quadrature. Our final results for the isovector average quark momentum fraction and the isovector helicity and transversity moments are given by $\langle x\rangle_{u-d}=0.180(25)(\genfrac{}{}{0pt}{2}{+14}{-06})\langle x\rangle_{\Delta u-\Delta d}=0.221(25)(\genfrac{}{}{0pt}{2}{+10}{-00})\langle x\rangle_{\delta u-\delta d}=0.212(32)(\genfrac{}{}{0pt}{2}{+20}{-10})$, respectively.

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