In this article we investigate harmonicity, Laplacians, mean value theorems and related topics in the context of quaternionic analysis. We observe that a Mean Value Formula for slice regular functions holds true and it is a consequence of the well known Representation Formula for slice regular functions over . Motivated by this observation, we have constructed three order-two differential operators in the kernel of which slice regular functions are, answering positively to the question: is a slice regular function over (analogous to an holomorphic function over ) “harmonic” in some sense, i.e. is it in the kernel of some order-two differential operator over ? Finally, some applications are deduced, such as a Poisson Formula for slice regular functions over and a Jensen’s Formula for semi-regular ones.

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