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In this paper, we study the dimension of cohomology of semipositive line bundles over Hermitian manifolds, and obtain an asymptotic estimate for the dimension of the space of harmonic -forms with values in high tensor powers of a semipositive line bundle when the fundamental estimate holds. As applications, we estimate the dimension of cohomology of semipositive line bundles on -convex manifolds, pseudo-convex domains, weakly -complete manifolds and complete manifolds. We also obtain the estimate of cohomology on compact manifolds with semipositive line bundles endowed with a Hermitian metric with analytic singularities and the related vanishing theorems.

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