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We analyze the Casimir effect for a flavor doublet of mixed scalar fields con- fined inside a one-dimensional finite region. In the framework of the unitary inequivalence between mass and flavor representations in quantum field theory, we employ two alternative approaches to derive the Casimir force: in the first case, the zero-point energy is evaluated for the vacuum of fields with definite mass, then similar calculations are performed for the vacuum of fields with defi- nite flavor. We find that signatures of mixing only appear in the latter context, showing the result to be independent of the mixing parameters in the former.

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