In this paper, we study the transition threshold problem for the 2-D Navier-Stokes equations around the Couette flow at large Reynolds number in a finite channel. We develop a systematic method to establish the resolvent estimates of the linearized operator and space-time estimates of the linearized Navier-Stokes equations. In particular, three kinds of important effects: enhanced dissipation, inviscid damping and boundary layer, are integrated into the space-time estimates in a sharp form. As an application, we prove that if the initial velocity satisfies $|v_0-(y, 0)|_{H^2}\le cRe^{-\frac 12}cRe$, then the solution of the 2-D Navier-Stokes equations remains within of the Couette flow for any time.

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