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In this paper, we give some results on closed polynomials and factorially closed polynomial in variables. In particular, we give a characterization of factorially closed polynomials in variables over an algebraically closed field for any characteristic. Furthermore, as an application of results on closed polynomials, we determine kernels of non-zero monomial derivations on the polynomial ring in two variables over a UFD. Finally, by using this result, for a field , we determine the non-zero monomial derivations on such that the quotient field of the kernel of is not equal to the kernel of in .

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