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Based on the quantum interference between two-identical-nucleus scattering at energies around the Coulomb barrier, the barrier positions for Ni+Ni and O+O are extracted from Mott oscillations in the angular distributions around 90 for the first time. The angle separation of pairs of Mott scattering valleys around 90 has a direct relationship with the closest distance between two nuclei in elastic scattering. Together with the barrier height from fusion excitation function, the extracted barrier position provides a sensitive probe to constrain the model predictions for the nucleus-nucleus potential barrier.

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