In this paper, we consider the following fractional Schrödinger equation \begin{equation*} \left{ \begin{array}{lcl} (-Δ)^{s}u+V(x)u=u^{{p_s}-ε}\ \ \ &\hbox{in}\ ^N,\ u>0\ \ \ &\hbox{in}\ ^N, \end{array} \right. \end{equation*} where , , , and is non-negative. We first use the Lyapunov-Schmidt reduction method to construct multi-peak solutions to the above equation provided that possesses stable critical points. Then we prove the non-degeneracy and local uniqueness of the multi-peak solutions, for , , via the blow-up argument based on various local Pohozaev identities. Due to the nonlocal property of the fractional Laplacian, we need to make delicate analysis of the approximate solutions and establish the local Pohozaev identities for the corresponding harmonic extension instead of . This approach not only requires to develop refined estimates for several integrals in the local Pohozaev identities, but also to apply Pohozaev identities through a markedly different way.

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