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We prove the mixed-norm Sobolev estimates for solutions to both divergence and non-divergence form time-dependent Stokes systems with unbounded measurable coefficients having small mean oscillations with respect to the spatial variable in small cylinders. As a special case, our results imply Caccioppoli’s type estimates for the Stokes systems with variable coefficients. A new -regularity criterion for Leray-Hopf weak solutions of Navier-Stokes equations is also obtained as a consequence of our regularity results, which in turn implies some borderline cases of the well-known Serrin’s regularity criterion.

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