One of the major goals of future Cosmic Microwave Background measurements is the accurate determination of the effective number of neutrinos . Reaching an experimental sensitivity of could indeed falsify the presence of any non-standard relativistic particles at $95 %$ c.l… In this paper, we test how this future constraint can be affected by the removal of two common assumptions: a negligible running of the inflationary spectral index and a precise determination of the neutron lifetime . We first show that the constraints on could be significantly biased by the unaccounted presence of a running of the spectral index. Considering the Stage-IV experiment, a negative running of ${\rm d}n/{\rm d}\ln k= - 0.002\Delta N_{\rm eff} = 0.03$. Moreover, given the current discrepancies between experimental measurements of the neutron lifetime , we show that the assumption of a conservative error of s could bring to a systematic error of . Complementary cosmological constraints on the running of the spectral index and a solution to the neutron lifetime discrepancy are therefore needed for an accurate and reliable future CMB bound of at percent level.

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