We report on precise measurements of absolute nonlinear ionization probabilities obtained by exposing optically trapped ultracold rubidium atoms to the field of an ultrashort laser pulse in the intensity range of $1 \times 10^{11}4 \times 10^{13}^2$. The experimental data are in perfect agreement with ab-initio theory, based on solving the time-dependent Schr"odinger equation without any free parameters. Ultracold targets allow to retrieve absolute probabilities since ionized atoms become apparent as a local vacancy imprinted into the target density, which is recorded simultaneously. We study the strong-field response of Rb atoms at two different wavelengths representing non-resonant and resonant processes in the demanding regime where the Keldysh parameter is close to unity.

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