The spectral radius of a graph is the largest eigenvalue of its adjacency matrix. Let be the family of connected graphs of spectral radius . We show that can be defined by a finite set of forbidden subgraphs if and only if $\lambda < \lambda^* := \sqrt{2+\sqrt{5}} \approx 2.058\lambda \not\in {\alpha_2, \alpha_3, \dots}\alpha_m = \beta_m^{1/2} + \beta_m^{-1/2}\beta_mx^{m+1}=1+x+\dots+x^{m-1}$. The study of forbidden subgraphs characterization for is motivated by the problem of estimating the maximum cardinality of equiangular lines in the -dimensional Euclidean space — a family of lines through the origin such that the angle between any pair of them is the same. Denote by the maximum number of equiangular lines in with angle . We establish the asymptotic formula $N_\alpha(n) = c_\alpha n + O_\alpha(1)\alpha \ge \frac{1}{1+2\lambda^*}$. In particular, and $N_{1/5}(n), N_{1/(1+2\sqrt{2})}(n) = \frac{3}{2}n+O(1)N_\alpha(n) \le 1.49n + O_\alpha(1)\alpha \neq \tfrac{1}{3}, \tfrac{1}{5}, \tfrac{1}{1+2\sqrt{2}}$, which improves a recent result of Balla, Dr"axler, Keevash and Sudakov.

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