An matrix on symbols is called -separating if for arbitrary pairwise disjoint column sets with for , there exists a row such that are also pairwise disjoint, where denotes the collection of components of restricted to row . Given integers and , denote by the maximal such that a corresponding matrix does exist. The determination of has received remarkable attentions during the recent years. The main purpose of this paper is to introduce two novel methodologies to attack the upper bound of . The first one is a combination of the famous graph removal lemma in extremal graph theory and a Johnson-type recursive inequality in coding theory, and the second one is the probabilistic method. As a consequence, we obtain several intriguing upper bounds for some parameters of , which significantly improve the previously known results.

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