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We present measurements of the Electronic Spin Resonance (ESR) of Nitrogen Vacancy (NV) centers in diamonds that are levitating in a ring Paul trap under vacuum. We observe ESR spectra of NV centers embedded in micron-sized diamonds at vacuum pressures down to mbar, limited by the green laser heating of the diamond. We also observe the NV photoluminescence at vacuum pressures down to mbar and discuss the steps required to control the NV spin under ultra-high vacuum.

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