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Deep inelastic scattering data on structure function obtained in the fixed-target experiments were analysed in the valence quark approximation with a next-to-next-to-leading-order accuracy. Parton distribution functions are parametrized by using information from the Gottfried sum rule. The strong coupling constant is found to be $\alpha_s(M_Z^2) = 0.1180 \pm 0.0020~\mbox(total,exp.error)$, which coincides very well with the average world value updated recently in a PDG report. The result for the second moment of the difference in and quark distributions is seen to be well compatible with the latest LATTICE result $<!!x!!>_{u-d}^{\rm LATTICE}=0.208 \pm 0.024$

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