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An matrix is said to have a self-interlacing spectrum if its eigenvalues , , are distributed as follows A method for constructing sign definite matrices with self-interlacing spectra from totally nonnegative ones is presented. We apply this method to bidiagonal and tridiagonal matrices. In particular, we generalize a result by O. Holtz on the spectrum of real symmetric anti-bidiagonal matrices with positive nonzero entries.

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