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In this paper, we build the unfolding approach from acyclic sign-skew-symmetric matrices of finite rank to skew-symmetric matrices of infinite rank, which can be regard as an improvement of that in the skew-symmetrizable case. Using this approach, we give a positive answer to the problem by Berenstein, Fomin and Zelevinsky in \cite{fz3} which asks whether an acyclic sign-skew-symmetric matrix is always totally sign-skew-symmetric. As applications, the positivity for cluster algebras in acyclic sign-skew-symmetric case is given; further, the -polynomials of cluster algebras are proved to have constant term 1 in acyclic sign-skew-symmetric case.

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