Comment on "Enhanced Diffusion of Enzymes that Catalyze Exothermic Reactions" by R.Golestanian

We (Riedel et al. Nature 2015), as well as others, have showed that some enzymes exhibit enhanced diffusion when active. In a recent PRL, (Golestanian, PRL 2015, arXiv:1508.03219) R.Golestanian theoretically examines a number of possible explanations for this phenomenon and concludes that “collective heating” is the best candidate to account for the observed diffusion coefficient increase. Here we present evidence showing that collective heating cannot possibly apply to our experiments.