Let and denote the set of all symmetric matrices over the ring of integers and the set of all orthogonal matrices over the field of rational numbers , respectively. The paper is mainly concerned with the following problem: Given a matrix . How can one find all rational orthogonal matrices such that $Q^TAQ\in {S_n()}Q^TAQ\in {S_n()}Q\in{O_n()}Q$ is \emph{a signed permutation matrix} (i.e., the matrix obtained from a permutation matrix by replacing each 1 in with 1 or )? A surprisingly simple answer was given in terms of whether the discriminant of the characteristic polynomial of is odd and square-free, which partially answers the above questions. More precisely, let $\Delta_A=\pm \res(\phi,\phi’)be \emph{the discriminant of matrixA}, where\res(\phi,\phi’)\phiA\phi’\Delta_A$ is odd and square-free, then with implies that is a signed permutation matrix. As an application, we present a simple and efficient method for testing whether a graph is determined by the generalized spectrum, which significantly extends our previous work.

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